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Patents

2007 MolCell Cover 2008 Curr Opinion MiCrobiol Cover

2014
  • Arenz, S., Ramu, H., Gupta, P., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Mankin, A.S., Wilson, D.N. (2014) Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide Nat. Commun. 5, 3501 DOI 10.138/ncomms4501.   
  • Vazquez-Laslop, N., Mankin, A.S. (2014) Protein accounting in the cellular economy Cell 157, 529-531.   
  • Sothiselvam, S., Liu, B., Han, W., Ramu, H., Klepacki, D., Atkinson, G.C., Brauer, A., Remm, M., Tenson, T., Schulten, K., Vázquez-Laslop, N., Mankin, A.S. (2014) Macrolide antibiotics allosterically predispose the ribosome for translation arrest. Proc Natl Acad Sci USA 111, 9804-9809.   
  • Martínez, A.K., Gordon, E., Sengupta, A., Shirole, N., Klepacki, D., Martinez-Garriga, B., Brown, L.M., Benedik, M.J., Yanofsky, C., Mankin, A.S., Vazquez-Laslop, N., Sachs, M.S., Cruz-Vera, L.R. (2014) Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan Nucl Acids Res 42, 1245–1256.   
  • Polikanov, Y.S., Szal, T., Jiang, F., Matsuda, R., Shiozuka, M., Steitz, T.A., Vazquez-Laslop, N., Mankin, A.S. (2014) Negamycin interferes with decoding and translocation by simultaneous interaction with rRNA and tRNA Mol Cell 56, XX-XX.   
  • Polikanov, Y.S., Osterman, I.A., Szal, T., Tashlitsky, V.N., Serebryakova, M.V., Kusochek, P., Bulkley, D., Malanicheva, I.A., Efimenko, T.A., Efremenkova, O.V., Konevega, A.L., Shaw, K.J., Bogdanov, A.A., Rodnina, M.V., Dontsova, O.A., Mankin, A.S., Steitz, T.A., Sergiev, P.V. (2014) Amicoumacin A inhibits translation by stabilizing mRNA interaction with the ribosome Mol Cell 56, XX-XX.   
  • Arenz, S., Meydan, S., Starosta, A., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Wilson, D.N. (2014) Drug sensing by the ribosome induces translational arrest via active site perturbation Mol Cell 56, XX-XX.   
  • Kannan, K., Kanabar, P., Schryer, D., Florin, T., Oh, E., Bahroos, N., Tenson, T., Weissman, J.S., Mankin, A.S. (2014) The general mode of translation inhibition by macrolide antibiotics Proc Natl Acad Sci USA 111, XXXXX-XXXXX.   
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2013
  • LaMarre, J., Mendes, R.E., Szal, T., Schwarz, S., Jones, R.N., Mankin, A.S. (2013) The genetic environment of the cfr gene and the presence of other mechanisms account for the very high linezolid resistance of Staphylococcus epidermidis isolate 426-3147L Antimicrob Agents Chemother 57, 1173-1179.   
  • Orelle, C., Szal, T., Klepacki, D., Shaw, K.J., Vázquez-Laslop, N., Mankin, A.S. (2013) Identifying the targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition Nucl Acids Res 10, 526.   
  • Gupta, P., Sothiselvam, S., Vázquez-Laslop, N., Mankin, A.S. (2013) Deregulation of translation due to post-transcriptional modification of rRNA explains why erm genes are inducible Nat Commun 4, 1984 DOI: 10.1038/ncomms2984.   
  • Orelle, C., Carlson, S., Kaushal, B., Almutairi, M.M., Liu, H., Ochabowicz, A., Quan, S., Pham, V.C., Squires, C.L., Murphy, B.T., Mankin, A.S. (2013) Tools for characterizing bacterial protein synthesis inhibitors. Antimicrob Agents Chemother 57, 5994-6004.   
  • Gupta, P., Kannan, K., Mankin, A.S., Vazquez-Laslop, N. (2013) Regulation of gene expression by macrolide-induced ribosomal frameshifting Mol Cell 52, 629-642.   
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2012
  • Locke JB, Rahawi S, Lamarre J, Mankin AS, Shaw KJ. (2012) Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05. Antimicrob Agents Chemother 56, 332-340.   
  • LeTourneau, N., Vimal, P., Klepacki, D., Mankin, A., Melman, A, (2012) Synthesis and antibacterial activity of desosamine-modified macrolide derivatives Bioorg Med Chem Lett 22, 4575-4578.   
  • Kannan, K., Vazquez-Laslop, N., Mankin, A.S. (2012) Selective protein synthesis by ribosomes with a drug-obstructed exit tunnel Cell 151, 508-520.   
  • Subramanian, S.L., Ramu, H., Mankin, A. S. (2012) Inducible Resistance to Macrolide Antibiotics Antibiotic Discovery and Development 1, 455-484.   
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2011
  • LaMarre, J. M, Howden, B. P., Mankin, A. S. (2011) Inactivation of the indigenous methyltransferase RlmN in Staphylococcus aureus increases linezolid resistance Antimicrob Agents Chemother 55, 2989-2991.   
  • LaMarre, J. M., Locke, J.B., Shaw, K.J., Mankin, A. S. (2011) Low Fitness Cost of the Multidrug Resistance Gene cfr Antimicrob Agents Chemother 55, 3714-3719.   
  • Vázquez-Laslop, N., Mankin, A.S. (2011) Picky nascent peptides do not talk to foreign ribosomes. Proc Natl Acad Sci USA 108, 5931-5932.   
  • Vázquez-Laslop, N., Klepacki, D., Mulhearn, D.C., Ramu, H., Krasnykh, O., Franzblau, S., Mankin, A.S. (2011) Role of antibiotic ligand in nascent peptide-dependent ribosome stalling. Proc Natl Acad Sci USA 108, 10496-10501.   
  • Vázquez-Laslop, N., Ramu, H., Mankin, A. S. (2011) Nascent peptide-mediated ribosome stalling promoted by antibiotics Ribosomes Structure Function and Dynamics 1, 377-392.   
  • Kannan, K., Mankin, A.S (2011) Macrolide antibiotics in the ribosome exit tunnel: species-specific binding and action Ann NY Acad Sci 1241, 33-47.   
  • Ramu, H. Nora Vazquez-Laslop, N., Klepacki, D., Dai, Q., Piccirilli, J., Micura, R. and Mankin, A.S. (2011) Nascent peptide in the ribosome exit tunnel affects functional properties of the A-Site of the peptidyl transferase center Mol Cell 41, 321-330.   
  • Ramu, H., Vázquez-Laslop, N., Klepacki, D., Dai, Q., Piccirilli, J., Micura, R., and Mankin, A.S. (2011) Nascent peptide in the ribosome exit tunnel affects functional properties of the A-Site of the peptidyl transferase center Mol Cell 41, 321-330.   
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2010
  • Yan, F., LaMarre, J. M., Röhrich, R., Wiesner, J., Jomaa, H., Mankin, A. S., Fujimori, D. G. (2010) RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA J Am Chem Soc 132, 3953-3964.   
  • Auerbach, T., Mermershtain, I., Davidovich, C., Bashan, A., Belousoff, M., Wekselman, I., Zimmerman, E., Xiong, L., Klepacki, D., Arakawa, K., Kinashi, H., Mankin, A.S., Yonath, A. (2010) The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics. Proc Natl Acad Sci USA 107, 1983-1988.   
  • David-Eden, H., Mankin, A. S., Mandel-Gurfreund, Y. (2010) Structural signatures of antibiotic binding sites on the ribosome Nucl Acids Res 38, 5982-5994.   
  • Vázquez-Laslop, N., Ramu, H., Klepacki, D., Kannan, K., Mankin, A. S. (2010) The key function of a conserved and modified rRNA residue in the ribosomal response to the nascent peptide. EMBO J 29, 3108-3117.   
  • Llano-Sotelo, B., Dunkle, J., Klepacki, D., Zhang, W., Fernandes, P., Cate, J. H. D., Mankin, A. S. (2010) Binding and action of CEM-101, a new fluoroketolide antibiotic that inhibits protein synthesis Antimicrob Agents Chemother 54, 4961-4970.   
  • Dunkle, J. A., Xiong, L., Mankin, A. S., Cate, J. H. D. (2010) Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action Proc Natl Acad Sci USA 107, 17152-17157.   
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2009
  • T. Siibak, L. Peil, L. Xiong, A. Mankin, J. Remme, T. Tenson (2009) Erythromycin- and chloramphenicol-induced ribosomal assembly defects are secondary effects of protein synthesis inhibition Antimicrob Agents Chemother 53, 563-571.   
  • H. Ramu, A. Mankin, N. Vazquez-Laslop (2009) Programmed drug-dependent ribosome stalling Mol Microbiol 71, 811-824.   
  • Llano-Sotelo, B., Klepacki, D., Mankin, A.S. (2009) Selection of short peptides, inhibitors of translation J Mol Biol 391, 813-819.   
  • Llano-Sotelo, B., Hickerson, R. P., Lancaster, L., Noller, H.F., Mankin, A.S. (2009) Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding RNA 15, 1597-1604.   
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2008
  • N. Vazquez-Laslop, C. Thum, A. S. Mankin (2008) Molecular mechanism of drug-dependent ribosome stalling Mol Cell 30, 190-202.   
  • S. M. Toh, L. Xiong, T. Bae, A. S. Mankin (2008) The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA RNA 14, 98-106.   
  • S. M. Toh, A. S. Mankin (2008) An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors J Mol Biol 380, 593-597.   
  • L. K. Smith, A. S. Mankin (2008) Transcriptional and translational control of the mlr operon, which confers resistance to seven classes of protein synthesis inhibitors Antimicrob Agents Chemother 52, 1703-1712.   
  • S. Samant, H. Lee, M. Ghassemi, J. Chen, J. L. Cook, A. S. Mankin, A. A. Neyfakh (2008) Nucleotide biosynthesis is critical for growth of bacteria in human blood PLoS Pathog 4, e37.   
  • A. S. Mankin, N. Polacek (2008) SPARK: a new peptidyl transferase activity assay Methods Mol Med 142, 107-116.   
  • A. S. Mankin (2008) Macrolide myths Curr Opin Microbiol 11, 414-421.   
  • M. Bailey, T. Chettiath, A. S. Mankin (2008) Induction of erm(C) expression by noninducing antibiotics Antimicrob Agents Chemother 52, 866-874.   
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2007
  • A. Yassin, A. S. Mankin (2007) Potential new antibiotic sites in the ribosome revealed by deleterious mutations in RNA of the large ribosomal subunit J Biol Chem 282, 24329-24342.   
  • S. M. Toh, L. Xiong, C. A. Arias, M. V. Villegas, K. Lolans, J. Quinn, A. S. Mankin (2007) Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid Mol Microbiol 64, 1506-1514.   
  • K. L. Leach, S. M. Swaney, J. R. Colca, W. G. McDonald, J. R. Blinn, L. M. Thomasco, R. C. Gadwood, D. Shinabarger, L. Xiong, A. S. Mankin (2007) The site of action of oxazolidinone antibiotics in living bacteria and in human mitochondria Mol Cell 26, 393-402.   
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2006
  • T. Tenson, A. Mankin (2006) Antibiotics and the ribosome Mol Microbiol 59, 1664-1677.   
  • D. Sherman, L. Xiong, A. S. Mankin, A. Melman (2006) Synthesis and biological investigation of new 4''-malonyl tethered derivatives of erythromycin and clarithromycin Bioorg Med Chem Lett 16, 1506-1509.   
  • A. S. Mankin (2006) Nascent peptide in the "birth canal" of the ribosome Trends Biochem Sci 31, 11-13.   
  • A. Mankin (2006) Antibiotic blocks mRNA path on the ribosome Nat Struct Mol Biol 13, 858-860.   
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2005
  • A. Yassin, K. Fredrick, A. S. Mankin (2005) Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics Proc Natl Acad Sci U S A 102, 16620-16625.   
  • L. Xiong, Y. Korkhin, A. S. Mankin (2005) Binding site of the bridged macrolides in the Escherichia coli ribosome Antimicrob Agents Chemother 49, 281-288.   
  • N. Polacek, A. S. Mankin (2005) The ribosomal peptidyl transferase center: structure, function, evolution, inhibition Crit Rev Biochem Mol Biol 40, 285-311.   
  • B. A. Maguire, A. D. Beniaminov, H. Ramu, A. S. Mankin, R. A. Zimmermann (2005) A protein component at the heart of an RNA machine: the importance of protein l27 for the function of the bacterial ribosome Mol Cell 20, 427-435.   
  • M. D. Erlacher, K. Lang, N. Shankaran, B. Wotzel, A. Huttenhofer, R. Micura, A. S. Mankin, N. Polacek (2005) Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451 Nucleic Acids Res 33, 1618-1627.   
  • M. Beringer, C. Bruell, L. Xiong, P. Pfister, P. Bieling, V. I. Katunin, A. S. Mankin, E. C. Bottger, M. V. Rodnina (2005) Essential mechanisms in the catalysis of peptide bond formation on the ribosome J Biol Chem 280, 36065-36072.   
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2004
  • V. Vimberg, L. Xiong, M. Bailey, T. Tenson, A. Mankin (2004) Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel Mol Microbiol 54, 376-385.   
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2003
  • K. A. Ruggero, L. K. Schroeder, P. C. Schreckenberger, A. S. Mankin, J. P. Quinn (2003) Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs Diagn Microbiol Infect Dis 47, 511-513.   
  • N. Polacek, M. J. Gomez, K. Ito, L. Xiong, Y. Nakamura, A. Mankin (2003) The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination Mol Cell 11, 103-112.   
  • M. Gaynor, A. S. Mankin (2003) Macrolide antibiotics: binding site, mechanism of action, resistance Curr Top Med Chem 3, 949-961.   
  • J. R. Colca, W. G. McDonald, D. J. Waldon, L. M. Thomasco, R. C. Gadwood, E. T. Lund, G. S. Cavey, W. R. Mathews, L. D. Adams, E. T. Cecil, J. D. Pearson, J. H. Bock, J. E. Mott, D. L. Shinabarger, L. Xiong, A. S. Mankin (2003) Cross-linking in the living cell locates the site of action of oxazolidinone antibiotics J Biol Chem 278, 21972-21979.   
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2002
  • P. Sander, L. Belova, Y. G. Kidan, P. Pfister, A. S. Mankin, E. C. Bottger (2002) Ribosomal and non-ribosomal resistance to oxazolidinones: species-specific idiosyncrasy of ribosomal alterations Mol Microbiol 46, 1295-1304.   
  • N. Polacek, S. Swaney, D. Shinabarger, A. S. Mankin (2002) SPARK--a novel method to monitor ribosomal peptidyl transferase activity Biochemistry 41, 11602-11610.   
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2001
  • L. Xiong, N. Polacek, P. Sander, E. C. Bottger, A. Mankin (2001) pKa of adenine 2451 in the ribosomal peptidyl transferase center remains elusive RNA 7, 1365-1369.   
  • T. Tenson, A. S. Mankin (2001) Short peptides conferring resistance to macrolide antibiotics Peptides 22, 1661-1668.   
  • N. Polacek, M. Gaynor, A. Yassin, A. S. Mankin (2001) Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide Nature 411, 498-501.   
  • P. A. Mann, L. Xiong, A. S. Mankin, A. S. Chau, C. A. Mendrick, D. J. Najarian, C. A. Cramer, D. Loebenberg, E. Coates, N. J. Murgolo, F. M. Aarestrup, R. V. Goering, T. A. Black, R. S. Hare, P. M. McNicholas (2001) EmtA, a rRNA methyltransferase conferring high-level evernimicin resistance Mol Microbiol 41, 1349-1356.   
  • G. Garza-Ramos, L. Xiong, P. Zhong, A. Mankin (2001) Binding site of macrolide antibiotics on the ribosome: new resistance mutation identifies a specific interaction of ketolides with rRNA J Bacteriol 183, 6898-6907.   
  • M. Bocchetta, L. Xiong, S. Shah, A. S. Mankin (2001) Interactions between 23S rRNA and tRNA in the ribosomal E site RNA 7, 54-63.   
  • L. Belova, T. Tenson, L. Xiong, P. M. McNicholas, A. S. Mankin (2001) A novel site of antibiotic action in the ribosome: interaction of evernimicin with the large ribosomal subunit Proc Natl Acad Sci U S A 98, 3726-3731.   
  • A. S. Mankin (2001) Ribosomal Antibiotics Mol Biol 35, 509-520.   
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2000
  • L. Xiong, P. Kloss, S. Douthwaite, N. M. Andersen, S. Swaney, D. L. Shinabarger, A. S. Mankin (2000) Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action J Bacteriol 182, 5325-5331.   
  • I. V. Velichutina, J. Dresios, J. Y. Hong, C. Li, A. Mankin, D. Synetos, S. W. Liebman (2000) Mutations in helix 27 of the yeast Saccharomyces cerevisiae 18S rRNA affect the function of the decoding center of the ribosome RNA 6, 1174-1184.   
  • S. I. Novikova, A. M. Bushueva, L. A. Trachuk, G. E. Konstantinova, A. V. Serkina, C. Hoischen, J. Gumpert, G. G. Chestukhina, A. Mankin, A. B. Shevelev (2000) Introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on Bacillus subtilis and provides temporary erythromycin protection in Proteus mirabilis FEMS Microbiol Lett 182, 213-218.   
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1999
  • L. Xiong, S. Shah, P. Mauvais, A. S. Mankin (1999) A ketolide resistance mutation in domain II of 23S rRNA reveals the proximity of hairpin 35 to the peptidyl transferase centre Mol Microbiol 31, 633-639.   
  • T. Tenson, J. V. Herrera, P. Kloss, G. Guarneros, A. S. Mankin (1999) Inhibition of translation and cell growth by minigene expression J Bacteriol 181, 1617-1622.   
  • A. S. Mankin, S. W. Liebman (1999) Baby, don't stop! Nat Genet 23, 8-10.   
  • P. Kloss, L. Xiong, D. L. Shinabarger, A. S. Mankin (1999) Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center J Mol Biol 294, 93-101.   
  • P. Khaitovich, T. Tenson, A. S. Mankin, R. Green (1999) Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive RNA 5, 605-608.   
  • P. Khaitovich, T. Tenson, P. Kloss, A. S. Mankin (1999) Reconstitution of functionally active Thermus aquaticus large ribosomal subunits with in vitro-transcribed rRNA Biochemistry 38, 1780-1788.   
  • P. Khaitovich, A. S. Mankin, R. Green, L. Lancaster, H. F. Noller (1999) Characterization of functionally active subribosomal particles from Thermus aquaticus Proc Natl Acad Sci U S A 96, 85-90.   
  • P. Khaitovich, A. S. Mankin (1999) Effect of antibiotics on large ribosomal subunit assembly reveals possible function of 5 S rRNA J Mol Biol 291, 1025-1034.   
  • N. S. Chernyaeva, E. J. Murgola, A. S. Mankin (1999) Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA J Bacteriol 181, 5257-5262.   
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1998
  • S. Tripathi, P. S. Kloss, A. S. Mankin (1998) Ketolide resistance conferred by short peptides J Biol Chem 273, 20073-20077.   
  • B. T. Porse, I. Leviev, A. S. Mankin, R. A. Garrett (1998) The antibiotic thiostrepton inhibits a functional transition within protein L11 at the ribosomal GTPase centre J Mol Biol 276, 391-404.   
  • M. Bocchetta, L. Xiong, A. S. Mankin (1998) 23S rRNA positions essential for tRNA binding in ribosomal functional sites Proc Natl Acad Sci U S A 95, 3525-3530.   
  • A. L. Arkov, A. Mankin, E. J. Murgola (1998) An rRNA fragment and its antisense can alter decoding of genetic information J Bacteriol 180, 2744-2748.   
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1997
  • T. Tenson, L. Xiong, P. Kloss, A. S. Mankin (1997) Erythromycin resistance peptides selected from random peptide libraries J Biol Chem 272, 17425-17430.   
  • A. S. Mankin (1997) Pactamycin resistance mutations in functional sites of 16 S rRNA J Mol Biol 274, 8-15.   
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1996
  • T. Tenson, A. DeBlasio, A. Mankin (1996) A functional peptide encoded in the Escherichia coli 23S rRNA Proc Natl Acad Sci U S A 93, 5641-5646.   
  • G. T. Tan, A. DeBlasio, A. S. Mankin (1996) Mutations in the peptidyl transferase center of 23 S rRNA reveal the site of action of sparsomycin, a universal inhibitor of translation J Mol Biol 261, 222-230.   
  • M. Dam, S. Douthwaite, T. Tenson, A. S. Mankin (1996) Mutations in domain II of 23 S rRNA facilitate translation of a 23 S rRNA-encoded pentapeptide conferring erythromycin resistance J Mol Biol 259, 1-6.   
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1995
  • T. Tenson, A. Mankin (1995) Comparison of functional peptide encoded in the Escherichia coli 23S rRNA with other peptides involved in cis-regulation of translation Biochem Cell Biol 73, 1061-1070.   
  • H. F. Noller, R. Green, G. Heilek, V. Hoffarth, A. Huttenhofer, S. Joseph, I. Lee, K. Lieberman, A. Mankin, C. Merryman, et al. (1995) Structure and function of ribosomal RNA Biochem Cell Biol 73, 997-1009.   
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Patents
  • Method of Screening to Find New Antibiotics. U.S. Patent 5,958,695 (1999). Inventor: Alexander Mankin
  • Mapping New Sites of Antibiotic Action in the Ribosome. International Publication Number WO 2006/121703. Inventors: Alexander Mankin and Aymen Yassin
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